P-082 Estradiol levels in idiopathic male infertility

Abstract Study question Investigating estradiol in relation with other sperm parameters might reveal new correlations which would help our understanding of male idiopathic infertility Summary answer Statistical significance was found a series E2 levels and sperm, hormonal health What is known already Male rising major concern, counts decreasing by 50-60% the recent decades. The causes for are very different (genetic, systemic, neurological, infections, trauma, etc.), but at least 44% cases idiopathic. Estradiol (E2) regulates spermatogenesis binding to classical intracellular estrogen receptors or membrane trigger genomic nongenomic signal transduction pathways. Although doesn’t carry any prognostic value itself, as its don’t vary significantly between infertile fertile men, association may lead better design, size, duration design cross-sectional study comprising 111 subjects- 87 patients (median: 34 years, range 20–55 years) 24 control 33 20–45 years). subjects were included this during 3 years (between October 2019- September2022). Inclusion criteria: spermatic according WHO 2010 Standards Exclusion Radiotherapy and/or pelvic chemotherapy (over last 6 months), genetic aberrations, endocrine diseases, urogenital bilateral orchiectomy, vasectomy occupational exposure. Participants/materials, setting, methods Infertile men grouped azoospermia (AZOO), oligoasthenoteratozoospermia (OATS/OATSS) oligoasthenozoospermia (OAS/OASS). Blood semen samples collected measured both seminal blood samples, along (sperm count, motility, morphology) factors (BMI, hormone vitamin D levels, BsmI polymorphism receptor gene) evaluated. assayed using electrochemiluminescence method RocheCobas E601 autoanalyzer. Main results role chance varies slightly patients’ groups, highest values being OAT/OATSS group. BMI higher group; significant OAS/OASS (p = 0.002) OATS/OATS 0.017) groups. analysis has revealed difference serum each group (AZOO p 0.0355; OATS/OATSS 0.0156; 0.0009), also 0.0366). E2/Inhibin ratio presented 0.0031) 0.0059). lowest E2/Vitamin detected groups 0.0254), A increase level observed from case homozygous mutant allele 0.0419). bring evidence regarding infertility. obtained data indicates an interplay Inhibin B highlighting it’s Limitations, reasons caution could be extended larger population sample, order further validate Wider implications findings activity plays important fertility investigated serve potential markers diagnosis management Trial registration number PN-III-P2-2.1-PED-2019-4402